Status of insecticide resistance and biochemical characterization of chlorpyrifos resistance in Sogatella furcifera (Hemiptera:Delphacidae) in Sichuan Province, China

• Published in: Pesticide biochemistry and physiology 2021-01, Vol.171, p.104723, Article 104723
• Main Authors: Ruan, Yanwei, Wang, Xuegui, Xiang, Xin, Xu, Xiang, Guo, Yaqi, Liu, Yinghong, Yin, Yong, Wu, Yaqiong, Cheng, Qinghua, Gong, Changwei, Zhang, Yuming, Hasnain, Ali, Shen, Litao, Jiang, Chunxian, Jiang, Surong
• Format: Article
• Language: English
• Subjects: Animals; China; Chlorpyrifos; Chlorpyrifos - toxicity; Cytochrome P450; Hemiptera - genetics; Insecticide resistance; Insecticide Resistance - genetics; Insecticides - toxicity; RNA interference; Sogatella furcifera
• ISSN: 0048-3575; 1095-9939
• DOI: 10.1016/j.pestbp.2020.104723

The white-backed planthopper, Sogatella furcifera (Horváth) (Hemiptera, Delphacidae), is an energetic rice insect pest in rice production or rice-growing areas. Due to excessive use of the chemical insecticide, S. furcifera has produced the high resistance to some frequently used insecticides. In this paper, the resistance levels of S. furcifera from the eight different areas of Sichuan Province against the five chemicals were monitored by using the rice seedling dipping during 2017–2018 to understand the resistance levels. The results showed that most of all populations have developed low or moderate level of resistance for chlorpyrifos (3.4 to 44.3-fold) and thiamethoxam (3.9- to 15.5-fold), the populations in the LS (1.7 to 5.4- fold)and WS (1.6 to 5.0- fold) regions were still sensitive or low resistance levels compared with other local populations. Almost all populations displayed the susceptible to imidacloprid (0.9- to 5.0-fold), buprofezin (0.9- to 4.3-fold) or low levels of resistance to pymetrozine (1.5- to 6.8-fold). The synergism experiment indicated that P450 enzymes may be important contributed to the metabolic detoxification of chlorpyrifos. The cross-resistance bioassay showed that there was no cross-resistance between chlorpyrifos and triflumezopyrim, but for sulfoxaflor, in the XY17 population. The relative expression level of twelve insecticide resistant-related P450 genes were analyzed by using qRT-PCR and found that CYP4C77, CYP418A1, CYP418A2, CYP408A3 and CYP6ER4 were significantly more expressed in the 3rd-instar nymph of the XY17 and XY18 field populations. To determine the main resistant-related P450 gene for chlorpyrifos, the relative expression level of five P450 genes were detected by using qRT-PCR from the G2 and G4 generation of XY17 under the pressure with LC50 of chlorpyrifos. The results showed that CYP6ER4 was significantly up-regulated expression in XY17 G2 and G4 generations population over 700-fold (P