Protective effect of rutin isolated from Spermococe hispida against cobalt chloride-induced hypoxic injury in H9c2 cells by inhibiting oxidative stress and inducing apoptosis

• Published in: Phytomedicine (Stuttgart) 2018-12, Vol.51, p.196-204
• Main Authors: Sundaram.R, Lakshmi, Sali, Veeresh Kumar, Vasanthi, Hannah R.
• Format: Article
• Language: English
• Subjects: Animals; Antioxidant; Antioxidants - pharmacology; Apoptosis - drug effects; Cardioprotection; Cell Hypoxia - drug effects; Cell Line; Cell Survival - drug effects; Cobalt; Hypoxia; Myocytes, Cardiac - drug effects; Oxidative stress; Oxidative Stress - drug effects; Plant Components, Aerial - chemistry; Protective Agents - pharmacology; Rats; Rubiaceae - chemistry; Rutin; Rutin - pharmacology; Spermococe hispida
• ISSN: 0944-7113; 1618-095X
• DOI: 10.1016/j.phymed.2018.09.229

Cardiovascular disease and its related deaths are increasing in the modern world. Therefore, there is a need to identify a plant based nutraceutical supplement with potent activity. Reportedly, the protective effect of the rutin in hypoxia-induced cardiomyocytes is due to the activation of molecular networks related to programmed cell death. Phytochemical methods and advanced analytical methods were employed to isolate natural products from Spermococe hispida their effects in cardiomyocyets. We reports herein that CoCl2-induced hypoxic condition significantly decreased cell viability as evidenced by MTT assay and cell cycle analysis. Western blot studies revealed an up-regulation of HIF-1α, BAX and caspase and down-regulation of BCl-2 expression, followed by modulation of Akt, p-Akt, p38 and p-p38. The oxidative abnormalities were ameliorated by rutin pretreatment, as deduced by the reduced CoCl2-induced cytotoxicity, MDA concentration and LDH activity and the enhanced levels of GSH and SOD in a dose-dependent manner. Rutin protects H9c2 cells from CoCl2-induced hypoxic damage by mitigating oxidative stress and preserving cell viability by modulating the antiapoptotic proteins. The overall findings reinforce the cardioprotective action of rutin, a potential source of antioxidant of natural origin, which may help in mitigating the progress of oxidative stress in hypoxic conditions such as myocardial infarction and stroke. [Display omitted]