Development of sorbents for immunoaffinity extraction of isoflavonoids

A set of immunosorbents specific for selected isoflavonoids has been prepared for the clean-up and concentration of plant extracts. Purified extracts were analyzed with HPLC–ESI-MS. This method is suitable for detection of isoflavonoid precursors or some accumulated isoflavonoid metabolites. [Displa...

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Bibliographic Details
Published in:Phytochemistry letters 2011-06, Vol.4 (2), p.113-117
Main Authors: Prokudina, E.A., Lanková, P., Koblovská, R., Al-Maharik, N., Lapčík, O.
Format: Article
Language:English
Subjects:
ELISA
HPLC–ESI-MS
Immunoaffinity chromatography
Immunoextraction
Immunosorbents
Isoflavonoid
Moraceae
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Summary:A set of immunosorbents specific for selected isoflavonoids has been prepared for the clean-up and concentration of plant extracts. Purified extracts were analyzed with HPLC–ESI-MS. This method is suitable for detection of isoflavonoid precursors or some accumulated isoflavonoid metabolites. [Display omitted] ► Immunoaffinity chromatography (IAC) can be used for the clean-up and the concentration of complex biological matrices, such as plant extracts. ► A set of immunosorbents specific for selected isoflavonoids has been prepared. ► An off-line combination of isoflavonoid-specific IAC and HPLC–ESI-MS is used for detection of isoflavonoids in non-leguminous plants. The majority of currently known isoflavonoids has been detected in and isolated from the Leguminosae; however, their presence has also been reported in numerous non-legumes. Detection of these secondary metabolites in non-legumes is impeded by the complex matrix of plant material and low concentration of the analytes. In this study, we present a combination of immunoaffinity chromatography (IAC) and HPLC–ESI-MS as a new tool for the detection of isoflavonoids. Immunoglobulin fractions of polyclonal rabbit antisera against conjugates of bovine serum albumin (BSA) with selected isoflavonoids (i.e. biochanin A, genistein and daidzein) were immobilized on a commercially available Affi-Gel 10 support. Immunosorbents were characterized according to their specificity and binding capacity and used in an off-line combination with HPLC–ESI-MS for a pilot study of isoflavonoid presence in Maclura pomifera and Ficus carica (Moraceae).
ISSN:1874-3900
1876-7486
DOI:10.1016/j.phytol.2010.12.005