Quantitative analysis of sesquiterpene lactones and thymol derivatives in extracts from Telekia speciosa

•An HPLC–DAD method was developed to quantify major terpenoids of Telekia speciosa.•Seven lactones and one thymol derivative were quantified in T. speciosa tissues.•Plants of different origin showed various capabilities to accumulate terpenoids.•Each organ of T. speciosa plant is characterized by sp...

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Bibliographic Details
Published in:Phytochemistry letters 2015-03, Vol.11, p.378-383
Main Authors: Stojakowska, Anna, Malarz, Janusz, Kisiel, Wanda
Format: Article
Language:English
Subjects:
Core–shell column
HPLC–DAD
Inulae
Sesquiterpene lactones
Telekia
Thymol derivatives
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Summary:•An HPLC–DAD method was developed to quantify major terpenoids of Telekia speciosa.•Seven lactones and one thymol derivative were quantified in T. speciosa tissues.•Plants of different origin showed various capabilities to accumulate terpenoids.•Each organ of T. speciosa plant is characterized by specific metabolite profile. A high-performance liquid chromatographic method based on a gradient elution and the application of core–shell type stationary phase was developed to estimate contents of sesquiterpene lactones and monoterpenoid thymol derivatives in the tissues of Telekia speciosa. The detection and quantification limits of the analytes were 0.05–0.29μgml−1 and 0.15–0.89μgml−1, respectively. Calibration curves showed good linearities (R2>0.9996) within the test ranges. Intra-day and inter-day precisions were satisfactory with RSD
ISSN:1874-3900
1876-7486
DOI:10.1016/j.phytol.2014.10.025