Dereplication-guided isolation of a new flavonoid triglycoside from Macroptilium martii and its cytotoxicity evaluation

• Published in: Phytochemistry letters 2020-10, Vol.39, p.144-150
• Main Authors: Souza, Ranna Beatris de Lima, do Nascimento, Yuri Mangueira, Gouveia, Rawny Galdino, Souto, Augusto Lopes, Sobral, Marianna Vieira, Costa, Vicente Carlos O., de Melo, José Iranildo Miranda, Silva, Marcelo Sobral, Tavares, Josean Fechine
• Format: Article
• Language: English
• Subjects: Cytotoxic activity; Fabaceae; Flavonoids; HPLC-MS/MS; Macroptilium martii
• ISSN: 1874-3900; 1876-7486
• DOI: 10.1016/j.phytol.2020.08.001

[Display omitted] •Isolation of a new flavonoid from Macroptilium martii.•Chemical and pharmacological study of a genus not well studied by the Fabaceae family.•Optimization of results by guided isolation using hyphenated techniques.•Potential cytotoxicity activity of many isolated compounds. The chemical investigation of Macroptilium martii (Fabaceae) has provided the identification of twelve compounds obtained by liquid chromatography coupled to mass spectrometry (LC–MS/MS), among which, a new flavonoid: isorhamnetin-3-O-galactose-6′'-O-rhamnose-4′”-O-galactose-2-methylbutyric acid (10) and two other flavonoids already reported. The structural elucidation of the new compound was carried out by HR-ESI-MS spectrometric analysis, 1H, 13C and two-dimensional NMR. Cytotoxic activity of 10 was evaluated towards HCT-116 (colorectal carcinoma), HeLa (cervical carcinoma), SK-MEL-28 (melanoma), PC-3 (prostate cancer) and MDA-MB-231 (breast cancer) cells.