Components involved in RNA–protein interaction at the 3′ untranslated region of rbcL mRNA of Populus deltoides

• Published in: Plant science (Limerick) 2004-10, Vol.167 (4), p.765-772
• Main Authors: Srivastava, Sandhya, Trivedi, Prabodh K, Nath, Pravendra
• Format: Article
• Language: English
• Subjects: 3'-untranslated region; 3′ UTR; Analytical, structural and metabolic biochemistry; Binding and carrier proteins; Biological and medical sciences; Chloroplast gene expression; Fundamental and applied biological sciences. Psychology; messenger RNA; molecular sequence data; nucleotide sequences; plant proteins; Populus deltoides; Proteins; rbcL protein; RNA processing; RNA-binding proteins; Stem-loop
• ISSN: 0168-9452; 1873-2259
• DOI: 10.1016/j.plantsci.2004.05.013

RNA–protein interactions occurring at the 3′ untranslated region (3′ UTR) of the mRNA of rbcL gene from Populus deltoides have been examined. The 3′ UTR of rbcL gene in P. deltoides is highly AT rich and contains invert repeat (IR) sequences capable of forming two stem-loops. Gel mobility shift assays followed by SDS–PAGE analysis revealed that the 3′ IR-RNA region interacts with a set of proteins having molecular weights between 66 and 26 kDa. The protein complex protected an approximately 82-nucleotide segment of 3′ UTR of rbcL mRNA when treated with RNase T1. An internal competition assay using variable lengths of the rbcL 3′ UTR indicated that the RNaseT1 protected segment included both stem-loops and a few sequences downstream including a conserved AU rich box.