Identification of stress-induced genes from the drought tolerant semi-arid legume crop horsegram ( Macrotyloma uniflorum (Lam.) Verdc.) through analysis of subtracted expressed sequence tags

Abiotic stresses adversely affect the agricultural productivity worldwide. Horsegram ( Macrotyloma uniflorum (Lam.) Verdc.) is a legume crop that can tolerate severe adverse environmental conditions such as drought, salinity and heavy metal contamination. As a first step towards characterization of...

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Bibliographic Details
Published in:Plant science (Limerick) 2008-09, Vol.175 (3), p.372-384
Main Authors: Reddy, P. Chandra Obul, Sairanganayakulu, G., Thippeswamy, M., Reddy, P. Sudhakar, Reddy, M.K., Sudhakar, Chinta
Format: Article
Language:English
Subjects:
abiotic stress
arid lands
Biological and medical sciences
complementary DNA
DNA libraries
drought
Drought stress
drought tolerance
expressed sequence tags
Expressed sequenced tags
Fundamental and applied biological sciences. Psychology
Gene expression
gene expression regulation
Genes. Genome
Horsegram
legumes
Macrotyloma uniflorum
messenger RNA
Molecular and cellular biology
Molecular genetics
plant stress
plant-water relations
soil water content
Subtractive hybridization
suppression subtractive hybridization
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Summary:Abiotic stresses adversely affect the agricultural productivity worldwide. Horsegram ( Macrotyloma uniflorum (Lam.) Verdc.) is a legume crop that can tolerate severe adverse environmental conditions such as drought, salinity and heavy metal contamination. As a first step towards characterization of genes that contribute to combating abiotic stresses, construction and analysis of subtracted cDNA library is reported here. Using this strategy a total of 1050 ESTs were isolated, sequenced, 959 high quality ESTs were obtained and clustered. Further, our analysis revealed that of these 531 sequences are unique and 30% of these have no homology to known proteins in the database. This observation has great relevance since horsegram is a stress-adapted legume crop. Further, to validate the identified differentially expressed genes, expression profiles of selected clones were analyzed using reverse-northern, northern blot analysis and we show that indeed these clones are differentially expressed under various abiotic stress conditions. The implications of the analyzed genes in abiotic stress tolerance also discussed.
ISSN:0168-9452
1873-2259
DOI:10.1016/j.plantsci.2008.05.012