Expression profiles and hormonal regulation of tobacco NtEXGT gene and its involvement in abiotic stress response

• Published in: Plant physiology and biochemistry 2017-02, Vol.111, p.203-215
• Main Authors: Kuluev, Bulat, Mikhaylova, Elena, Berezhneva, Zoya, Nikonorov, Yuri, Postrigan, Bogdan, Kudoyarova, Guzel, Chemeris, Aleksey
• Format: Article
• Language: English
• Subjects: Abscisic acid; Abscisic Acid - pharmacology; Acetates - pharmacology; Adaptation, Physiological - drug effects; Adaptation, Physiological - genetics; ARGOS-LIKE; Auxin; Cadmium - toxicity; Cell expansion; Cold Temperature; Cyclopentanes - pharmacology; Gene Expression Profiling; Gene Expression Regulation, Plant - drug effects; Genes, Plant; Nicotiana - drug effects; Nicotiana - genetics; Nicotiana - physiology; Nicotiana tabacum L; Oxylipins - pharmacology; Phylogeny; Plant Growth Regulators - pharmacology; Plant Leaves - drug effects; Plant Leaves - metabolism; Plants, Genetically Modified; Pyridones - pharmacology; RNA, Messenger - genetics; RNA, Messenger - metabolism; Seedlings - drug effects; Seedlings - genetics; Sodium Chloride - pharmacology; Stress tolerance; Stress, Physiological - drug effects; Stress, Physiological - genetics; Xyloglucan endotransglucosylases/hydrolases
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2016.12.005

Despite the intensive study of xyloglucan endotransglucosylases/hydrolases, their multifaceted role in plant growth regulation in changing environmental conditions is not yet clarified. The functional role of the large number of genes encoding this group of enzymes is also still unclear. NtEXGT gene encodes one of xyloglucan endotransglucosylases/hydrolases (XTHs) of Nicotiana tabacum L. The highest level of NtEXGT gene expression was detected in young flowers and leaves near the shoot apex. Expression of the NtEXGT gene in leaves was induced by cytokinins, auxins, brassinosteroids and gibberellins. NtEXGT gene was also up-regulated by salinity, drought, cold, cadmium and 10 μM abscisic acid treatments and down-regulated in response to 0 °C and 100 μM abscisic acid. Pretreatment of leaves with fluridone contributed to smaller increase in the level of NtEXGT transcripts in response to drought stress. These data suggest that NtEXGT gene is ABA-regulated and probably implicated in ABA-dependent signaling in response to stress factors. 35S::NtEXGT plants of tobacco showed higher rate of root growth under salt-stress conditions, greater frost and heat tolerance as compared with the wild type tobacco plants. •NtEXGT expression depends on stress factors.•NtEXGT expression mainly regulated by auxins and ABA.•Constitutive expression of NtEXGT gene enhances stress tolerance.