Copper toxicity affects phosphorus uptake mechanisms at molecular and physiological levels in Cucumis sativus plants

• Published in: Plant physiology and biochemistry 2020-12, Vol.157, p.138-147
• Main Authors: Feil, Sebastian B., Pii, Youry, Valentinuzzi, Fabio, Tiziani, Raphael, Mimmo, Tanja, Cesco, Stefano
• Format: Article
• Language: English
• Subjects: Copper - toxicity; Cucumber; Cucumis sativus - drug effects; Cucumis sativus - metabolism; Gene expression; Gene Expression Regulation, Plant; Phenotype; Phosphorus; Phosphorus - metabolism; Plant Roots - metabolism; Soil; Soil Pollutants - toxicity; Transporters
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2020.10.023

Due to the deliberate use of cupric fungicides in the last century for crop-defence programs, copper (Cu) has considerably accumulated in the soil. The concentrations of Cu often exceed the safety limits of risk assessment for Cu in soil and this may cause toxicity in plants. Copper toxicity induces nutritional imbalances in plants and constraints to plants growth. These aspects might be of paramount importance in the case of phosphorus (P), which is an essential plant macronutrient. In this work, hydroponically grown cucumber plants were used to investigate the influence of the exposure to different Cu concentrations (0.2, 5, 25 and 50 μM) on i) the phenotypic traits of plants, particularly at root level, ii) the nutrient content in both roots and shoots, and iii) the P uptake mechanisms, considering both the biochemical and molecular aspects. At high Cu concentrations (i.e. above 25 μM), the shoot and root growth resulted stunted and the P influx rate diminished. Furthermore, two P transporter genes (i.e. CsPT1.4 and CsPT1.9) were upregulated at the highest Cu concentration, albeit with different induction kinetics. Overall, these results confirm that high Cu concentrations can limit the root acquisition of P, most likely via a direct action on the uptake mechanisms (e.g. transporters). However, the alteration of root plasma membrane permeability induced by Cu toxicity might also play a pivotal role in the observed phenomenon. •Plants exposed to Cu toxicity modify the architecture of the root system.•Excessive Cu availability hinders Pi influx in intact cucumber roots.•Cu toxicity induced P deficiency-like response in cucumber plants.•Plants exposed to Cu toxicity upregulate the expression of Pi transporters.