Lower air humidity reduced both the plant growth and activities of photosystems I and II under prolonged heat stress

• Published in: Plant physiology and biochemistry 2023-01, Vol.194, p.246-262
• Main Authors: Lysenko, Eugene A., Kozuleva, Marina A., Klaus, Alexander A., Pshybytko, Natallia L., Kusnetsov, Victor V.
• Format: Article
• Language: English
• Subjects: Air humidity; Barley; Chlorophyll; Chlorophyll fluorescence; Heat stress; Heat-Shock Response; Hot Temperature; Humidity; Maize; P700 light absorption; Photosystem I Protein Complex - metabolism; Photosystem II Protein Complex - metabolism; Plant Leaves - metabolism; Plants - metabolism; Water
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2022.11.016

The warming is global problem. In natural environments, heat stress is usually accompanied by drought. Under drought conditions, water content decreases in both soil and air; yet,the effect of lower air humidity remains obscure. We supplied maize and barley plants with an unlimited source of water for the root uptake and studied the effect of relative air humidity under heat stress. Young plants were subjected for 48 h to several degrees of heat stress: moderate (37 °C), genuine (42 °C), and nearly lethal (46 °C). The conditions of lower air humidity decreased the photochemical activities of photosystem I and photosystem II. The small effect was revealed in the control (24 °C). Elevating temperature to 37 °C and 42 °C increased the relative activities of both photosystems; the photosystem II was activated more. Probably, this is why the effect of air humidity disappeared at 37 °C; the small inhibiting effect was observed at 42 °C. At 46 °C, lower air humidity substantially magnified the inhibitory effect of heat. As a result, the maximal and relative activities of both photosystems decreased in maize and barley; the photosystem II was inhibited more. Under the conditions of 46 °C at lower air humidity, the plant growth was greatly reduced. Maize plants increased water uptake by roots and survived; barley plants were unable to increase water uptake and died. Therefore, air humidity is an important component of environmental heat stress influencing activities of photosystem I and photosystem II and thereby plant growth and viability under severe stress conditions. •Maize and barley survived 48 h at 46 °C in hydroponics under continuous illumination.•Heat stress at 37 °C and 42 °C increased relative activities of photosystem I and II.•Lower air humidity decreased activities of photosystem I and II.•Lower air humidity decreased the activity of photosystem II more than photosystem I.•At 46 °C, lower air humidity magnified inhibition of photosystems and plant growth.