Lung production of platelet-activating factor acetylhydrolase in oleic acid-induced acute lung injury

• Published in: Prostaglandins, leukotrienes and essential fatty acids leukotrienes and essential fatty acids, 2007-07, Vol.77 (1), p.1-8
• Main Authors: Salluh, Jorge I, Pino, Alexandre V, Silva, Adriana R, Gomes, Rachel N, Souza, Heitor S, e Silva, Jose Roberto Lapa, Jandre, Frederico C, Giannella-Neto, Antonio, Zimmerman, Guy A, Stafforini, Diana M, Prescott, Stephen M, Castro-Faria-Neto, Hugo C, Bozza, Patrícia T, Bozza, Fernando A
• Format: Article
• Language: English
• Subjects: 1-Alkyl-2-acetylglycerophosphocholine Esterase - biosynthesis; 1-Alkyl-2-acetylglycerophosphocholine Esterase - blood; Advanced Basic Science; Animals; Biological and medical sciences; Bronchoalveolar Lavage Fluid - chemistry; Endocrinology & Metabolism; Female; Fundamental and applied biological sciences. Psychology; Immunohistochemistry; Kinetics; Lung - enzymology; Lung - metabolism; Medical sciences; Oleic Acid; Pneumology; Respiratory Distress Syndrome, Adult - chemically induced; Respiratory Distress Syndrome, Adult - enzymology; Respiratory system : syndromes and miscellaneous diseases; Swine; Time Factors; Vertebrates: endocrinology
• ISSN: 0952-3278; 1532-2823
• DOI: 10.1016/j.plefa.2007.04.001

Abstract Platelet-activating factor (PAF) is a proinflammatory mediator that plays a central role in acute lung injury (ALI). PAF- acetylhydrolases (PAF-AHs) terminate PAF's signals and regulate inflammation. In this study, we describe the kinetics of plasma and bronchoalveolar lavage (BAL) PAF-AH in the early phase of ALI. Six pigs with oleic acid induced ALI and two healthy controls were studied. Plasma and BAL samples were collected every 2 h and immunohistochemical analysis of PAF-AH was performed in lung tissues. PAF-AH activity in BAL was increased at the end of the experiment (BAL PAF-AH Time 0=0.001±0.001 nmol/ml/min/g vs Time 6=0.031±0.018 nmol/ml/min/g, p =0.04) while plasma activity was not altered. We observed increased PAF-AH staining of macrophages and epithelial cells in the lungs of animals with ALI but not in healthy controls. Our data suggest that increases in PAF-AH levels are, in part, a result of alveolar production. PAF-AH may represent a modulatory strategy to counteract the excessive pro-inflammatory effects of PAF and PAF-like lipids in lung inflammation.