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Differences in the expression profile of endo-β-(1,6)-d-galactanase in pathogenic and non-pathogenic races of Colletotrichum lindemuthianum grown in the presence of arabinogalactan, xylan or Phaseolus vulgaris cell walls

The scope of this study was the isolation and molecular characterization of endo-β-1,6-galactanase (ebg) from pathogenic (1472) and non-pathogenic (0) races of Colletotrichum lindemuthianum cultivated with arabinogalactan, xylan or Phaseolus vulgaris cell walls as carbon sources. Characterization of...

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Bibliographic Details
Published in:Physiological and molecular plant pathology 2017-08, Vol.99, p.75-86
Main Authors: Villa-Rivera, Maria G., Zavala-Páramo, María G., Conejo-Saucedo, Ulises, López-Romero, Everardo, Lara-Márquez, Alicia, Cano-Camacho, Horacio
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Language:English
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Summary:The scope of this study was the isolation and molecular characterization of endo-β-1,6-galactanase (ebg) from pathogenic (1472) and non-pathogenic (0) races of Colletotrichum lindemuthianum cultivated with arabinogalactan, xylan or Phaseolus vulgaris cell walls as carbon sources. Characterization of the ebg cDNA and 3D protein modeling revealed typical elements of the GH30 family of galactanases. The growth of both races with glucose showed basal transcription levels of ebg. When glucose was replaced with arabinogalactan, xylan or plant cell walls, ebg transcription markedly increased in race 1472 but not in race 0. Putative DNA-binding sites for Cre, Xlnr, ACEI, PacC and Gal4 transcriptional factors were predicted in ebg genes from Colletotrichum species. •The ebg cDNA and protein 3D modeling revealed the typical elements of galactanases of family GH30.•The ebg gene showed differential expression in pathogenic and non-pathogenic races.•The enzyme EBG has a role in establishment of the infection.•The ebg genes present putative DNA binding sites for Cre, Xlnr, ACEI, PacC and Gal4.
ISSN:0885-5765
1096-1178
DOI:10.1016/j.pmpp.2016.10.002