Modes of action of squamocin in the anal papillae of Aedes aegypti larvae

• Published in: Physiological and molecular plant pathology 2018-01, Vol.101, p.172-177
• Main Authors: Costa, Marilza da Silva, de Paula, Sergio Oliveira, Martins, Gustavo Ferreira, Zanuncio, José Cola, Santana, Antônio Euzébio Goulart, Serrão, José Eduardo
• Format: Article
• Language: English
• Subjects: Acetogenin; Aquaporin; Membrane transport; V-type-H+-ATPase; Yellow fever
• ISSN: 0885-5765; 1096-1178
• DOI: 10.1016/j.pmpp.2017.04.001

The morphological and the primary transcript levels of the genes for aquaporin AaAQP4 and the membrane transporter AaV-H+-ATPase in the anal papillae of A. aegypti larvae exposed to squamocin were evaluated. Squamocin caused great vacuolization in the anal papillae wall, basal lamina disruption with loss of canalicular spaces, and disorganization of the cuticular layers. There was also decrease of AaAQP4 transcript levels and inhibition of AaV-H+-ATPase in the anal papillae of larvae. Morphological together with decrease in the AaAQP4 and AaV-H+-ATPase primary transcript levels suggest that squamocin may affect osmoregulation and ion-regulation of this insect followed by death. [Display omitted] •Finding of alternative methods of chemical control of the insect vector Aedes aegypti are necessary.•Squamocin affects the morphology and primary transcript of genes for water and ions flow in anal papillae of Aedes aegypti.•Squamocin is a bioactive molecule that could be used to develop sustainable control for this insect vector.