Histological and biochemical aspects of compatible and incompatible wheat- Puccinia striiformis interactions

The resistant, slow and fast-rusting cultivars were selected from 15 wheat cultivars to study biochemical and histological aspects of compatible and incompatible wheat-Puccinia striiformis interactions. Final rust severity (%) and area under disease progress curve were significantly decreased in res...

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Bibliographic Details
Published in:Physiological and molecular plant pathology 2019-04, Vol.106, p.120-128
Main Authors: Esmail, Samar.M., Omara, Reda.I., Abdelaal, Khaled.A.A., Hafez, Yaser.M.
Format: Article
Language:English
Subjects:
Antioxidant enzymes
Electron microscope
Puccinia striiformis
Reactive oxygen species
ROS
Wheat
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Summary:The resistant, slow and fast-rusting cultivars were selected from 15 wheat cultivars to study biochemical and histological aspects of compatible and incompatible wheat-Puccinia striiformis interactions. Final rust severity (%) and area under disease progress curve were significantly decreased in resistant cultivars compared with susceptible ones. Biochemical analysis revealed that reactive oxygen species (ROS), i.e. superoxide (O2•-) and hydrogen peroxide (H2O2) significantly accumulated in the susceptible cultivars. While, activities of catalase (CAT), peroxidase (POX) and chlorophyll concentrations were increased in resistant cultivars. Histological examination using scanning and transmission electron microscope indicated that approximately all haustoria and hyphae were inhibited in resistant cultivar (Misr-1), development of haustorial mother cells and haustoria were retarded, and they were abnormal. On the other hand, large amounts of intercellular hyphae presented in the intercellular spaces and many haustoria were produced in fast-rusting cultivar (Sids-12). Nevertheless, a little of sours and spores on upper epidermis and in mesophyll tissue were observed in slow-rusting cultivar (Sakha-93).. •Wheat cultivars Sids-12 and Gemmieza-7 were resistant but now became susceptible as a result of dynamic changes of Puccinia striiformis f. sp. tritici.•Chlorophyll concentration was increased in resistant cultivars.•Electrolyte leakage and ROS were decreased however, enzymes activity were increased in resistant cultivars.•Scanning and transmission electron microscope examination indicated that approximately all hyphae were inhibited in resistant cultivar compared with fast and slow-rusting cultivars.
ISSN:0885-5765
1096-1178
DOI:10.1016/j.pmpp.2018.11.004