Improved synthesis of P(3HB- co-3HV- co-3HHx) terpolymers by mutant Cupriavidus necator using the PHA synthase gene of Chromobacterium sp. USM2 with high affinity towards 3HV

Unlike polyhydroxyalkanoates (PHAs) copolymers, the controlled and efficient synthesis of PHA terpolymers from triglycerides and fatty acids are yet to be established. This study demonstrates the production of P(3HB- co-3HV- co-3HHx) terpolymer with a wide range of 3HV monomer compositions from mixt...

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Bibliographic Details
Published in:Polymer degradation and stability 2010-08, Vol.95 (8), p.1436-1442
Main Authors: Bhubalan, Kesaven, Rathi, Devi-Nair, Abe, Hideki, Iwata, Tadahisa, Sudesh, Kumar
Format: Article
Language:English
Subjects:
3-Hydroxyhexanoate
3-Hydroxyvalerate
Applied sciences
Bioconversions. Hemisynthesis
Biological and medical sciences
Biotechnology
Chromobacterium sp
Crude palm kernel oil
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Organic polymers
Physicochemistry of polymers
Polycondensation
Preparation, kinetics, thermodynamics, mechanism and catalysts
Sodium valerate
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Summary:Unlike polyhydroxyalkanoates (PHAs) copolymers, the controlled and efficient synthesis of PHA terpolymers from triglycerides and fatty acids are yet to be established. This study demonstrates the production of P(3HB- co-3HV- co-3HHx) terpolymer with a wide range of 3HV monomer compositions from mixtures of crude palm kernel oil and 3HV precursors using a mutant Cupriavidus necator PHB −4 transformant harboring the PHA synthase gene ( phaC) of a locally isolated Chromobacterium sp. USM2. The PHA synthase of Chromobacterium has an unusually high affinity towards 3HV monomer. P(3HB- co-3HV- co-3HHx) terpolymers with 3HV monomer composition ranging from 2 to 91 mol% were produced. Generation of 3HHx monomers was affected by the concentration and feeding time of 3HV precursor. P(3HB- co-24 mol% 3HV- co-7 mol% 3HHx) exhibited mechanical properties similar to that of common low-density polyethylene. P(3HB- co-3HV- co-3HHx) terpolymers with a wide range of 3HV molar fraction had been successfully synthesized by adding lower concentrations of 3HV precursors and using a PHA synthase with high affinity towards 3HV monomer.
ISSN:0141-3910
1873-2321
DOI:10.1016/j.polymdegradstab.2009.12.018