On the comparison between diafiltration and isothermal titration calorimetry: Determination of the amount of analytes bound to water-soluble polymers

Aqueous systems composed of low molecular-weight species (LMWS) and water-soluble polymers (WSP) present important technological applications. Therefore, continuous advances on characterization techniques are crucial to gain a better understanding on how these systems interact and subsequently furth...

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Bibliographic Details
Published in:Polymer testing 2019-07, Vol.76, p.443-447
Main Authors: Orozco, Felipe, Redondo-Gómez, Carlos, Vega-Baudrit, José Roberto, Moreno-Villoslada, Ignacio
Format: Article
Language:English
Subjects:
Diafiltration
Isothermal titration calorimetry
Low molecular-weight species
Molecular interactions
Water-soluble polymers
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Summary:Aqueous systems composed of low molecular-weight species (LMWS) and water-soluble polymers (WSP) present important technological applications. Therefore, continuous advances on characterization techniques are crucial to gain a better understanding on how these systems interact and subsequently further modulate their properties by tuning their preparation process. For that matter, this work aims at comparing a developing characterization tool, diafiltration (DF), with a well-established method for studying LMWS/macromolecule interactions, isothermal titration calorimetry (ITC). Chlorpheniramine maleate (CPM) and poly(sodium 4-styrenesulfonate) (PSS) are used as a model aqueous LMWS/WSP system. The comparison is based on the CPM fraction bound to PSS measured by both methods. The values obtained are (0.89 ± 0.00) and (0.88 ± 0.05) for DF and ITC, respectively. The similarity between these values is noteworthy considering the dissimilarity of the process on which both techniques are based (filtration and heat exchange). This work constitutes a first step on setting DF and ITC as complementary and supporting techniques. Additional implications from these results are further discussed. •The amount of analytes bound to water-soluble polymers was determined by diafiltration and isothermal titration calorimetry.•The values obtained through both techniques are remarkably similar.•This points out the complementary potential of both techniques for characterizing polymer-analytes interaction.
ISSN:0142-9418
1873-2348
DOI:10.1016/j.polymertesting.2019.04.003