10 Oncostatin M regulates trophoblast invasion through STAT1 expression

Introduction Trophoblast cell invasion is one of key steps for successful pregnancy, which is controlled by several factors including cytokines that activate intracellular signaling cascades including the Janus kinase / signal transducer and activator of transcription (JAK-STAT) system. Objectives W...

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Bibliographic Details
Published in:Pregnancy hypertension 2016-07, Vol.6 (3), p.182-182
Main Authors: de Sousa, Francisco Lazaro Pereira, Chaiwangyen, Wittaya, Morales-Prieto, Diana M, Photini, Stella M, Ospina-Prieto, Stephanie, Sass, Nelson, Daher, Silvia, Markert, Udo R
Format: Article
Language:English
Subjects:
Cardiovascular
Obstetrics and Gynecology
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Summary:Introduction Trophoblast cell invasion is one of key steps for successful pregnancy, which is controlled by several factors including cytokines that activate intracellular signaling cascades including the Janus kinase / signal transducer and activator of transcription (JAK-STAT) system. Objectives We aimed to analyze the effect of cytokines on STAT1 expression and trophoblast cell functions. Methods Immortalized HTR-8/SVneo cells were treated with interleukin-6 (IL-6) IL-11, granulocyte macrophage colony-stimulating factor (GM-CSF), leukemia inhibitory factor (LIF) and oncostatin M (OSM). STAT1 phosphorylation (tyr-701) was analyzed by Western blotting and immunofluorescence staining. Simultaneously, cells were incubated with 50 μ M Fludarabine (specific STAT1 inhibitor) or small interfering RNA (siRNA) to inhibit the expression of STAT1 and followed by cytokine stimulation. STAT1 DNA-binding activity, cell viability and invasion were analyzed by DNA-binding, MTS and Matrigel invasion assays. Results Western blot analysis and immunofluorescence staining of STAT1 phosphorylation revealed that OSM induced phosphorylation of STAT-1 stronger than LIF in HTR-8/SVneo. IL-6, IL-11 and GM-CSF had no effect on STAT1 activation. STAT1 phosphorylation was inhibited by STAT1 siRNA and Fludarabine. STAT1 inhibition reduced STAT1 DNA binding activity and OSM could not rescue this effect. OSM stimulated HTR-8/SVneo cell viability and invasion, whereas STAT1 inhibition decreased trophoblast cell invasion. This reduction could not be prevented by OSM. Conclusions These findings demonstrate that the OSM-STAT1 axis plays an important role in trophoblastic cells and STAT1 activation may induce a variety of cell functions
ISSN:2210-7789
DOI:10.1016/j.preghy.2016.08.092