79 sFlt-1 and miRNA-210 expressions have strong correlation in preeclamptic placentas

• Published in: Pregnancy hypertension 2016-07, Vol.6 (3), p.216-216
• Main Authors: Korkes, Henri Augusto, de oliveira, Leandro Gustavo, Sass, Nelson, Ananth Karumanchi, S, Rajakumar, Augustine
• Format: Article
• Language: English
• Subjects: Cardiovascular; Obstetrics and Gynecology
• ISSN: 2210-7789
• DOI: 10.1016/j.preghy.2016.08.161

Introduction Preeclampsia (PE) is a multifactor disease responsible for enormous amount of maternal and perinatal mortality worldwide. The growing knowledge about the disease has highlighted several biomarkers that may act in synergistic ways in the pathogenesis of PE, mainly the anti-angiogenic factor sFlt-1. Recently, some authors have demonstrated the involvement of the angiogenic microRNA-210 (miR-210) in the development of PE. Objective Our aims for this study were to re-evaluate the expression of sFlt-1 and miR-210 by preeclamptic placentas and determine any correlation between these two biomarkers. Methods Placentas were collected from normal and preeclamptic patients ( n = 11 in each group). These tissue samples were flash-frozen in liquid nitrogen within half an hour of placental delivery. Total RNA was extracted using the acid guanidinium thiocyanate–phenol–chloroform extraction technique and their concentrations were determined using spectrophotometer. qRT-PCR was performed for the quantitation of Flt1, sFlt13, sFlt14 and miR-210. Results The mRNA expression of sFlt-1 and miR-210 in PE and controls were respectively (14.70–1.047) and (2.737–1.125). We also identified a strong correlation between sFlt-1 and miR-210 ( R2 = 0.7967, p < 0.0001). Conclusion Our results confirm previous observations regarding the increased mRNA expressions of sFlt-1 and miRNA-210 by preeclamptic placentas and highlight the dysregulation of the angiogenic process in the pathogenesis of PE.