Extraction and purification of phycocyanin from Calothrix sp

The extraction and purification of phycocyanin from Calothrix sp., cyanobacteria isolated from rice fields in Cuernavaca, Morelos, Mexico is described. Phycocyanin was extracted with 2 mg of lysozyme/g wet biomass, and purified by anion chromatography using Q-Sepharose fast-flow (Pharmacia ®, 1.5 cm...

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Bibliographic Details
Published in:Process biochemistry (1991) 2004-10, Vol.39 (12), p.2047-2052
Main Authors: Santiago-Santos, Ma.Carmen, Ponce-Noyola, Teresa, Olvera-Ramı́rez, Roxana, Ortega-López, Jaime, Cañizares-Villanueva, Rosa Olivia
Format: Article
Language:English
Subjects:
Calothrix sp
Cyanobacteria
Extraction
Lysozyme
Phycocyanin
Purification
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Summary:The extraction and purification of phycocyanin from Calothrix sp., cyanobacteria isolated from rice fields in Cuernavaca, Morelos, Mexico is described. Phycocyanin was extracted with 2 mg of lysozyme/g wet biomass, and purified by anion chromatography using Q-Sepharose fast-flow (Pharmacia ®, 1.5 cm×10 cm) column and hydrophobic interaction chromatography with methyl macro-prep (Bio-Rad ®, 1.5 cm×20 cm) column. The purified protein showed a p I of 5.2 and has two subunits with apparent molecular mass of 21–17 kDa each. The estimated molecular mass of native purified phycocyanin was 114 kDa, suggesting a stereochemistry of (αβ) 3.
ISSN:1359-5113
1873-3298
DOI:10.1016/j.procbio.2003.10.007