Fed-batch production of recombinant fuculose-1-phosphate aldolase in E. coli

Escherichia coli strain XL1 blue (pTrcfuc) was used to overexpress fuculose-1-phosphate aldolase (Fuc-1-phosphate) in a fed-batch bioreactor operating at constant specific growth rate, 0.1 h −1. Maximum dry cell weight concentration in the fermentor was 45 g dm −3, over this biomass concentration ox...

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Bibliographic Details
Published in:Process biochemistry (1991) 2005-02, Vol.40 (2), p.707-716
Main Authors: Durany, Olga, de Mas, Carles, López-Santı́n, Josep
Format: Article
Language:English
Subjects:
Chiral synthesis
CC bond
Escherichia coli
Fed-batch
Fuculose-1-phosphate aldolase
Recombinant protein
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Summary:Escherichia coli strain XL1 blue (pTrcfuc) was used to overexpress fuculose-1-phosphate aldolase (Fuc-1-phosphate) in a fed-batch bioreactor operating at constant specific growth rate, 0.1 h −1. Maximum dry cell weight concentration in the fermentor was 45 g dm −3, over this biomass concentration oxygen became limiting. Once the recombinant strain behaviour was characterised under fixed growth conditions, optimal induction conditions to maximise the recombinant protein productivity in the system was studied. Time after induction to achieve maximum specific activity was around 11 h. Best results obtained were: specific activity of 1100 AU g −1 DCW, enzyme concentration of 47,000 AU dm −3 (around 3 g dm −3) and global volumetric productivity of 1200 AU dm −3 h −1.
ISSN:1359-5113
1873-3298
DOI:10.1016/j.procbio.2004.01.058