Chemopreventive role of Tin oxide-Chitosan-Polyethylene glycol-Crocin nanocomposites against Lung cancer: An in vitro and in vivo approach

• Published in: Process biochemistry (1991) 2022-09, Vol.120, p.186-194
• Main Authors: Seshadri, Vidya Devanathadesikan, Oyouni, Atif Abdulwahab A., Hawsawi, Yousef M., Aljohani, Saad Ali S., M. Al-Amer, Osama, AlZamzami, Waseem, Mufti, Ahmad Hasan
• Format: Article
• Language: English
• Subjects: A549 cells; Crocin; Inflammation; Lung cancer; Nanocomposite
• ISSN: 1359-5113; 1873-3298
• DOI: 10.1016/j.procbio.2022.05.028

Lung cancer (LC) is a foremost cause of cancer-associated mortalities worldwide and it causes 17 % of the total cancer incidences and 20 % of the cancer-associated mortalities annually. In this exploration, we intended to formulate the Tin oxide-Chitosan-Polyethylene glycol-Crocin nanocomposites (SCP-Cr-NCs) and examine its anticancer role against the LC in both in vitro and in vivo models. The formulated SCP-Cr-NCs were characterized using several methods. The LC was initiated to the BALB/c mice via injecting the 2 × 106 of A549 cells into the posterior flank and treated with the 10 and 20 µM of formulated SCP-Cr-NCs for 19 days. The tumor weight and xenobiotic dysfunction markers status was examined by standard methods. The status of pro-inflammatory markers was studied using ELISA kits. The lung histology was examined microscopically. The viability of SCP-Cr-NCs treated A549 cells were investigated. The ROS and MMP status and apoptosis in the SCP-Cr-NCs treated A549 cells were inspected by fluorescence staining techniques. The tumor weight and the status of xenobiotic dysfunction markers were remarkably reduced by the SCP-Cr-NCs. The SCP-Cr-NCs supplementation also diminished the IL-1β, TNF-α, IL-6, and CEA. The viability of SCP-Cr-NCs administered A549 cells were reduced drastically. The outcomes of fluorescence assay was demonstrated that the SCP-Cr-NCs were diminished the MMP and elevated the ROS status and apoptosis in the A549 cells. SCP-Cr-NCs also restrained the cell adhesion of A549 cells. Collectively, our findings provided the evidence that the formulated SCP-Cr-NCs demonstrated the anticancer potential and it could be a talented chemopreventive candidate to treat the LC. [Display omitted] •Lung cancer (LC) is a major cause of cancer-associated mortalities worldwide.•Nano-formulations has aroused as a talented tool for cancer treatment.•Chitosan micelles was being emerged in the field of drug delivery.•The SCP-Cr-NCs has reduced the tumor weight and inflammatory markers in the LC mice.