Fabrication and characterization of doxorubicin conjugated bimetallic gold and platinum nanoparticles for human cancer cells and its cell death mechanism

• Published in: Process biochemistry (1991) 2024-02, Vol.137, p.111-121
• Main Authors: Chinnathambi, Arunachalam, Alharbi, Sulaiman Ali, Meganathan, Velmurugan, Muthusami, Sridhar, Arulselvan, Palanisamy
• Format: Article
• Language: English
• Subjects: Anticancer; Apoptosis; Bimetallic nanoparticles; Drug delivery; Phosphatidylserine
• ISSN: 1359-5113; 1873-3298
• DOI: 10.1016/j.procbio.2023.12.007

An environmentally friendly preparation technique was used to explore the mechanism of action of hybrid hydrophilic bimetallic gold-platinum (Au@Pt-NPs) against human cancer cell lines, followed by conjugation with doxorubicin (DOX). The synthesized DOX-loaded Au@Pt-NPs (DOX/Au@Pt-NPs) were examined using transmission electron microscopy, X-ray diffraction, and UV–vis spectroscopy to determine their physicochemical characteristics. Using a straightforward incubation method, phytochemicals-mediated capping efficiency and loading of DOX onto the Au@Pt-NPs surface (84%) was demonstrated by FT-IR spectroscopy. Studies on the release kinetics of DOX under acidic tumor conditions revealed a pH-regulated dependence response. According to the in vitro anticancer assessment, human cancer cells treated with DOX-loaded Au@Pt-NPs demonstrated a 3-fold increase in apoptosis compared to DOX treatment alone. Additionally, the DOX/Au@Pt-NPs nanocarrier demonstrated a time-dependent increase in phosphatidylserine flipping, an apoptosis induction marker, and a cell-specific reaction in HeLa and HepG2 cells, according to luminescent results from a real-time, bioluminescent recombinant annexin V test. According to the results, the manufacturing process is simple, and the hydrophilic Au@Pt-NPs were successful in DOX delivery and loading against human cancer cell lines, indicating their prospective as a supplementary for cervical and hepatocellular cancer therapy. [Display omitted] •We fabricated bimetallic gold-platinum (Au@Pt-NPs) conjugation with doxorubicin (DOX).•Synthesized DOX-loaded Au@Pt-NPs (DOX/Au@Pt-NPs) were examined using various spectral methods.•Studies on the release kinetics of DOX under acidic tumor conditions revealed a pH-regulated response.•Human cancer cells treated with DOX-loaded Au@Pt-NPs saw a 3-fold increase in apoptosis.•DOX/Au@Pt-NPs nanocarrier demonstrated a time-released phosphatidylserine exposure, an apoptosis induction.