Role of gamma radiation as an agent modulator of Mucor subtilissimus UCP1262 Fibrinolytic Enzyme (MsFE)

• Published in: Process biochemistry (1991) 2024-08, Vol.143, p.13-22
• Main Authors: Cardoso, Kethylen Barbara Barbosa, Nascimento, Thiago Pajeú, Correia, Patyanne Carvalho, da Silva Batista, Juanize Matias, Brandão-Costa, Romero Marcos Pedrosa, Ferreira, Kétura Rhammá Cavalcante, de Carvalho-Silva, Jônatas, da Cruz Augusto Neves Petersen, Maria Teresa, Albuquerque, Wendell, Salazar e Fernandes, Thiago de, Converti, Attilio, Porto, Ana Lúcia Figueiredo
• Format: Article
• Language: English
• Subjects: Fibrinolytic therapy; Gamma radiation; Protease activity
• ISSN: 1359-5113; 1873-3298
• DOI: 10.1016/j.procbio.2024.04.017

Thrombosis is a dangerous condition that can result in major damage. Fibrinolytic enzymes are significant therapeutic agents for thrombosis. This study focused on MsFE, a fibrinolytic protease from M. subtilissimus UCP1262, exploring its physicochemical traits and the impact of gamma radiation on its activity. Investigations included: fibrinogenolytic activity, effects of solvents and ions, gamma rays, enzyme shelf life, FTIR, CD, clotted times determination, and enzymatic kinetics. Notably, the major results obtained show the protease retained 90 % activity in organic solvents, FTIR indicated carbonyl functional groups and Circular dichroism spectroscopic studies indicated that the secondary structure (β-sheet) of enzyme remains mostly unaffected over pH 2.0–9.0, whereas considerable changes were observed in the tertiary structure. Kinetic analysis revealed altered parameters post-irradiation. Gamma radiation initially enhanced enzyme activity (10 kGy) but progressively decreased beyond 20 kGy. Metallic ions, especially magnesium, augmented irradiated enzyme activity. This study highlights gamma radiation's potential as a modulator for enhanced fibrinolytic activity, suggesting future biomedical applications. [Display omitted] •Mucor subtilissimus proves its significance as a producer of fibrinolytic proteases.•The protease degrades the α, β and γ chains of fibrinogen.•The 10 kGy radiation increased the activity of the enzyme by approximately 10 %.•Fe2+, Cu2+ and Mg2+ were able to increase the activity of the irradiated enzyme.•Produced enzymes can be a highly productive fibrinolytic for medical applications.