Preparation of a novel laccase-modified Fe3O4/TiO2 catalyst for simultaneous delignification and saccharification of Spartina alterniflora Loisel

The development of a cost-effective and environment-friendly conversion method to overcome the recalcitrance of biomass is a challenging task for enhancing the value-added potential of lignocellulosic compounds. In this study, a photo-enzyme composite catalyst by immobilizing laccase on Fe3O4/TiO2 (...

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Bibliographic Details
Published in:Process biochemistry (1991) 2024-11, Vol.146, p.387-400
Main Authors: Xiang, Yulin, Dai, Chunyu, Wang, Yefei, Zhang, Yongbo, Zhu, Jing, Men, Rongteng, Pang, Zhuoyi
Format: Article
Language:English
Subjects:
Delignification
Laccase
LC-Fe₃O₄/TiO₂ preparation
Photocatalytic pretreatment
Saccharification
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Summary:The development of a cost-effective and environment-friendly conversion method to overcome the recalcitrance of biomass is a challenging task for enhancing the value-added potential of lignocellulosic compounds. In this study, a photo-enzyme composite catalyst by immobilizing laccase on Fe3O4/TiO2 (denoted as LC-Fe3O4/TiO2) was applied to the pretreatment and enzymatic saccharification of Spartina alterniflora Loisel (SAL) under the action of alternating light/dark cycles. The effect of the pretreatment temperature, pretreatment time, LC-Fe3O4/TiO2 concentration, mass concentration of straw particles, and hydrolysis time on delignification efficiency and reducing sugar yield was investigated by a central composite design (CCD). Under the selected conditions (48 ℃, pretreatment time of 70 min, LC-Fe3O4/TiO2 concentration of 4.9 mg/mL, mass concentration of straw particles of 22 % (w/v), hydrolysis time of 55 h), 92.53 % delignification and 129.85 mg/g reducing sugar yield were achieved. The enzymatic hydrolysis efficiency was higher compared to conventional methods. Moreover, the LC-Fe3O4/TiO2 showed good recyclability and reusability. Developed LC-Fe3O4/TiO2 particles have a high potential for use in biomass utilization. [Display omitted] •Laccase-Fe3O4/TiO2 integrated catalyst (LC-Fe3O4/TiO2) is first synthesized.•Effects of LC-Fe3O4/TiO2 on pretreatment and saccharification is studied.•LC-Fe3O4/TiO2 can enhance sugar yield under the alternation of light and dark.•LC-Fe3O4/TiO2 could be efficiently reused up to 3 cycles.
ISSN:1359-5113
DOI:10.1016/j.procbio.2024.09.019