Amperometric Monitoring of the Neuropeptide “Substance-P” Level in Biological Fluids

Presented here is a capillary enzyme immunoassay constructed on a foil-based microfluidic chip for rapid determination of Substance-P (SP) level in human plasma, serum and saliva. Using a competitive ELISA implemented into the capillary, the amperometrically detected SP by flow injection analysis wa...

Full description

Saved in:
Bibliographic Details
Published in:Procedia chemistry 2009-09, Vol.1 (1), p.1275-1278
Main Authors: Horak, Josef, Enderle, Barbara, Bakirci, Hüseyin, Urban, Gerald A.
Format: Article
Language:English
Subjects:
amperometry
capillary immunoassay
immunosensor
microfluidic system
Substance-P
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Presented here is a capillary enzyme immunoassay constructed on a foil-based microfluidic chip for rapid determination of Substance-P (SP) level in human plasma, serum and saliva. Using a competitive ELISA implemented into the capillary, the amperometrically detected SP by flow injection analysis was related to the percentage amount between SP standard (or sample) and SP-labelled glucose oxidase conjugate bound to the capture antibody. A detection limit of 10 pg/ml was obtained. The assay allows fast reading of diagnostically relevant levels. Producing reliable results comparable to microplate format, the immunochip sensor may have a significant impact in point of care diagnostics.
ISSN:1876-6196
1876-6196
DOI:10.1016/j.proche.2009.07.318