Metabolic Engineering of Escherichia coli Cells for Ethanol Production under Aerobic Conditions

The ethanol production by recombinant Escherichia coli introducing of pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adhB) from Zymomonas mobilis was investigated under aerobic conditions. In aerobic culture (KLa = 1.5 min-1), the cells expressing pdc and adhB produced 0.4g l-1 ethanol when...

Full description

Saved in:
Bibliographic Details
Published in:Procedia chemistry 2015, Vol.16, p.600-607
Main Authors: Fithriani, Suryadarma, Prayoga, Mangunwidjaja, Djumali
Format: Article
Language:English
Subjects:
aerobic culture
Escherichia coli
ethanol production
formate dehydrogenase
NADH regeneration
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The ethanol production by recombinant Escherichia coli introducing of pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adhB) from Zymomonas mobilis was investigated under aerobic conditions. In aerobic culture (KLa = 1.5 min-1), the cells expressing pdc and adhB produced 0.4g l-1 ethanol when cultured for 18h. This value was improved in BW25113Δpta/pHfdh/pTadhB-pdc following 4g l-1 formate feeding at 0.8g l-1 ethanol. In higher oxygenation level (KLa = 6.1 min-1), the production of ethanol was further enhanced at 1.79g l-1 ± 0.37g l-1 after 24h cultivation. Formate was found not detectable at the end of culture, indicating complete degradation this organic acid to regenerate NADH from NAD+. The culture strategy was effective to inactivate lactate dehydrogenase, which is major competitor for ethanol production in utilizing NADH.
ISSN:1876-6196
1876-6196
DOI:10.1016/j.proche.2015.12.098