An enhanced platform for cell electroporation: controlled delivery and electrodes functionalization

This work presents an improved platform for single-site electroporation and controlled transfectants’ delivery. The device consists of a gold microelectrode array (MEA) with integrated microfluidics and nanostructured titanium dioxide (ns-TiO2) functionalized electrodes. Human cervical cancer cells...

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Bibliographic Details
Published in:Procedia engineering 2010, Vol.5, p.45-48
Main Authors: Odorizzi, L., Ress, C., Collini, C., Morganti, E., Lorenzelli, L., Coppedé, N., Alabi, A.B., Iannotta, S., Vidalino, L., Macchi, P.
Format: Article
Language:English
Subjects:
Cell electroporation
Functionalization
Microelectrode array (MEA)
Microfluidics
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Summary:This work presents an improved platform for single-site electroporation and controlled transfectants’ delivery. The device consists of a gold microelectrode array (MEA) with integrated microfluidics and nanostructured titanium dioxide (ns-TiO2) functionalized electrodes. Human cervical cancer cells (HeLa) have been successfully cultivated on chip surface. The system has been previously tested by electroporating them with Lucifer Yellow (LY) and then, in order to validate the approach and cell viability, with plasmids for the enhanced expression of Green Fluorescence Protein (pEGFP) delivered through microchannels.
ISSN:1877-7058
1877-7058
DOI:10.1016/j.proeng.2010.09.044