Sensitive Detection of Interferon-Gamma with Engineered Proteins and Surface Plasmon Resonance Biosensor

We present a novel surface plasmon resonance (SPR) biosensor for detection of human interferon gamma (hIFNγ), an important diagnostic marker for several infectious diseases. The biosensor employs engineered proteins derived from albumin binding domain (ABD) and selected with ribosome display to prov...

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Bibliographic Details
Published in:Procedia engineering 2011, Vol.25, p.940-943
Main Authors: Šípová, H., Ševců, V., Kuchař, M., Ahmad, Jawid N., Mikulecký, P., Šebo, P., Malý, P., Homola, J.
Format: Article
Language:English
Subjects:
Biosensor
Engineered proteins
Interferon gamma
Scaffold
Surface plasmon resonance
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Summary:We present a novel surface plasmon resonance (SPR) biosensor for detection of human interferon gamma (hIFNγ), an important diagnostic marker for several infectious diseases. The biosensor employs engineered proteins derived from albumin binding domain (ABD) and selected with ribosome display to provide high affinity towards hIFNγ. We demonstrate that the biosensor provides rapid, sensitive, and cost-effective platform for the detection of hIFNγ and allows its detection at nanomolar levels both in buffer and diluted human plasma.
ISSN:1877-7058
1877-7058
DOI:10.1016/j.proeng.2011.12.231