Comparison of two Analytical Methods (ELISA and LC-MS/MS) for Determination of Aflatoxin B1 in Corn and Aflatoxin M1 in Milk

The aim of this paper is to assess the closeness of agreement between results of ELISA and LC-MS/MS methods for determination of aflatoxin B1 in corn and aflatoxin M1 in milk. Samples of corn (n=100) and milk (n=250) were simultaneously analyzed using ELISA and LC-MS/MS methods, after the severe dro...

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Bibliographic Details
Published in:Procedia food science 2015, Vol.5, p.270-273
Main Authors: Stefanovic, Srdjan, Spiric, Danka, Petronijevic, Radivoj, Trailovic, Jelena Nedeljkovic, Milicevic, Dragan, Nikolic, Dragica, Jankovic, Sasa
Format: Article
Language:English
Subjects:
aflatoxin B1
aflatoxin M1
corn
ELISA
LC-MS/MS
milk
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Summary:The aim of this paper is to assess the closeness of agreement between results of ELISA and LC-MS/MS methods for determination of aflatoxin B1 in corn and aflatoxin M1 in milk. Samples of corn (n=100) and milk (n=250) were simultaneously analyzed using ELISA and LC-MS/MS methods, after the severe drought that affected Serbia in summer 2012 resulting in occurrence of aflatoxin B1 in corn and aflatoxin M1 in milk. Regression analysis showed higher level of agreement between aflatoxin B1 samples (R2=0.994), compared to aflatoxin M1 samples (R2=0.920). However, both techniques were satisfactory in meeting the requirements for official control purposes.
ISSN:2211-601X
2211-601X
DOI:10.1016/j.profoo.2015.09.077