Modification of radiation-induced oxidative damage in liposomal and microsomal membrane by eugenol

Radiation-induced membrane oxidative damage, and their modification by eugenol, a natural antioxidant, was investigated in liposomes and microsomes. Liposomes prepared with DPH showed decrease in fluorescence after γ -irradiation, which was prevented significantly by eugenol and correlated with magn...

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Bibliographic Details
Published in:Radiation physics and chemistry (Oxford, England : 1993) England : 1993), 2006-03, Vol.75 (3), p.384-391
Main Authors: Pandey, B.N., Lathika, K.M., Mishra, K.P.
Format: Article
Language:English
Subjects:
Antioxidants
Eugenol
Lipid peroxidation
Membrane oxidative damage
Phospholipase-C
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Summary:Radiation-induced membrane oxidative damage, and their modification by eugenol, a natural antioxidant, was investigated in liposomes and microsomes. Liposomes prepared with DPH showed decrease in fluorescence after γ -irradiation, which was prevented significantly by eugenol and correlated with magnitude of oxidation of phospholipids. Presence of eugenol resulted in substantial inhibition in MDA formation in irradiated liposomes/microsomes, which was less effective when added after irradiation. Similarly, the increase in phospholipase C activity observed after irradiation in microsomes was inhibited in samples pre-treated with eugenol. Results suggest association of radio- oxidative membrane damage with alterations in signaling molecules, and eugenol significantly prevented these membrane damaging events.
ISSN:0969-806X
1879-0895
DOI:10.1016/j.radphyschem.2005.09.011