Ovarian tissue features assessed in bovine fetuses after vitrification and xenotransplantation procedures

•Vitrification followed xenotransplantation decreased the follicular and blood vessel densities.•Both vitrification and xenotransplantation procedures induce the micronucleus formation in ovarian tissue cells. Cryopreservation and transplantation of ovarian tissue are proposed methods for the restor...

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Published in:Reproductive biology 2021-12, Vol.21 (4), p.100575, Article 100575
Main Authors: Moraes, Giovanna Faria de, Ronda, Juliano Bergamo, Campos, Bruna de Souza, Rocha, Carina Diniz, Soares, Mayara Mafra, Gowen, Blake Hampton, de Freitas, Ricardo Aurélio Avelar, Silva Junior, Elso Donizete, Marques, Jéssica Cristina dos Santos, Gontijo, Daniel Amaral, de Moraes, Ana Beatriz Almeida, Brito, Ruth Magalhães, de Oliveira Júnior, Robson José, Alves, Benner Geraldo, Alves, Kele Amaral, Santos, Ricarda Maria dos
Format: Article
Language:English
Subjects:
Chromosomal abnormalities
Preantral follicles
Stromal cells
Vascularization
Vitrification
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Summary:•Vitrification followed xenotransplantation decreased the follicular and blood vessel densities.•Both vitrification and xenotransplantation procedures induce the micronucleus formation in ovarian tissue cells. Cryopreservation and transplantation of ovarian tissue are proposed methods for the restoration of endocrine function and reproductive potential. Therefore, this study aimed to evaluate the effects of vitrification and xenotransplantation on follicle viability, activation, stromal cell integrity, vascularization, and micronuclei formation. Bovine fetal ovaries were fragmented and assigned to the following groups: Fresh control (FC), ovarian fragments immediately fixed; Vitrified control (VC), ovarian fragments vitrified; Vitrified xenotransplanted (VX), ovarian fragments vitrified and xenotransplanted; and Fresh xenotransplanted (FX), ovarian fragments xenotransplanted. Ovarian fragments were grafted in female BALB/c mice and recovered after 14 days. Follicular viability was preserved (P >  0.05) in VC group. The rate of developing follicles was greater (P 
ISSN:1642-431X
DOI:10.1016/j.repbio.2021.100575