Effect of lipopolysaccharide on developmental competence of oocytes

•LPS perturbed the nuclear maturation of bovine oocytes by inhibiting meiotic progression.•LPS decreased mitochondrial membrane potential in matured oocytes.•LPS altered the distribution pattern of active mitochondria in the oocyte cytoplasm.•Oocytes matured under LPS treatment showed decreased deve...

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Bibliographic Details
Published in:Reproductive toxicology (Elmsford, N.Y.) N.Y.), 2017-08, Vol.71, p.1-7
Main Authors: Magata, Fumie, Shimizu, Takashi
Format: Article
Language:English
Subjects:
Animals
Blastocyst - drug effects
Cattle
Cell Growth Processes - drug effects
DNA Copy Number Variations - drug effects
DNA, Mitochondrial - genetics
Embryonic Development - drug effects
Female
Inner cell mass
Lipopolysaccharide
Lipopolysaccharides - toxicity
Lymphocyte Antigen 96 - metabolism
Membrane Potential, Mitochondrial - drug effects
Mitochondria DNA
Oocyte maturation
Oocytes - cytology
Oocytes - drug effects
Oocytes - metabolism
Oocytes - physiology
Toll-Like Receptor 2 - metabolism
Toll-Like Receptor 4 - metabolism
Trophoblast
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Summary:•LPS perturbed the nuclear maturation of bovine oocytes by inhibiting meiotic progression.•LPS decreased mitochondrial membrane potential in matured oocytes.•LPS altered the distribution pattern of active mitochondria in the oocyte cytoplasm.•Oocytes matured under LPS treatment showed decreased development to the blastocyst stage. In postpartum dairy cows, lipopolysaccharide (LPS) derived from gram-negative bacteria such as Escherichia coli causes uterine inflammation resulting in low fertility. The aim of this study was to determine the effect of LPS on the developmental competence of bovine oocytes in vitro. LPS perturbed the nuclear maturation of bovine oocytes by inhibiting meiotic progression. Although LPS did not affect the copy number of mitochondrial DNA, it decreased mitochondrial membrane potential in matured oocytes. LPS inhibited mitochondrial redistribution throughout the cytoplasm. Oocytes matured under LPS treatment showed decreased development to the blastocyst stage. Moreover, the trophoblast cell number of blastocysts was significantly lower when the oocytes were matured in the presence of LPS. Our findings suggest that LPS might impair the nuclear and cytoplasmic maturation of oocytes and obstruct subsequent embryonic development in dairy cows.
ISSN:0890-6238
1873-1708
DOI:10.1016/j.reprotox.2017.04.001