A comparison of the effects of lindane and FeCl 3 /ADP on spontaneous contractions in isolated rat or human term myometrium

• Published in: Reproductive toxicology (Elmsford, N.Y.) N.Y.), 2017-12, Vol.74, p.164-173
• Main Authors: Fanning, Rebecca A, Campion, Deirdre P, O'Shea, Maeve, Carey, Michael F, O'Connor, John J
• Format: Article
• Language: English
• Subjects: Acetylcysteine - pharmacology; Adenosine Diphosphate - analogs & derivatives; Adenosine Diphosphate - toxicity; Adult; Animals; Antioxidants - pharmacology; Female; Humans; Insecticides - toxicity; Iron Chelating Agents - toxicity; Lindane - toxicity; Myometrium - drug effects; Myometrium - physiology; Rats, Wistar; Uterine Contraction - drug effects
• ISSN: 0890-6238; 1873-1708
• DOI: 10.1016/j.reprotox.2017.09.011

Oxidative stress affects the contractile behavior of smooth muscle resulting in complications during labor. Toxicants such as lindane and ferric chloride (FeCl )/adenosine diphosphate (ADP) cause oxidative stress and have previously been shown to inhibit smooth muscle contraction. In this study we examined the effects of the oxygen species scavengers, ascorbic acid and N-acetylcysteine on lindane and FeCl /ADP's inhibition of spontaneous myometrial contractions in rat and human myometrium. Lindane and FeCl /ADP gave rise to concentration-dependent reductions in rat (EC 11.8×10 M and 0.9×10 M) and human myometrial contractions (EC 16.3×10 M and 1.1×10 M, respectively). Pre-treatment with N-acetylcysteine significantly increased the EC for the effects of lindane on motility index of human tissue and reduced the maximum inhibitory effect of FeCl /ADP on contractions in both rat and human myometrium. Ascorbic acid reduced the effects of FeCl /ADP in rat tissue only. In conclusion pre-treatment with specific antioxidants may protect both rat and human myometrium from the inhibitory effects of lindane and FeCl /ADP.