Cyantraniliprole impairs reproductive parameters by inducing oxidative stress in adult female wistar rats

• Published in: Reproductive toxicology (Elmsford, N.Y.) N.Y.), 2022-01, Vol.107, p.166-174
• Main Authors: da Silva Scarton, Suellen Ribeiro, Tsuzuki, Felipe, Guerra, Marina Trevizan, dos Santos, Dayane Priscila, dos Santos, Aldair Casagrande, Guimarães, Ana Tereza Bittencourt, Simão, Andréa Name Colado, Beu, Célia Cristina Leme, Fernades, Glaura Scantamburlo Alves
• Format: Article
• Language: English
• Subjects: Animals; Cholinesterases - metabolism; Endocrine Disruptors - toxicity; Estrous cycle; Estrous Cycle - drug effects; Female; Glutathione Transferase - metabolism; Insecticide; Insecticides - toxicity; ortho-Aminobenzoates - toxicity; Ovaries; Ovary - drug effects; Ovary - metabolism; Ovary - pathology; Oxidative Stress - drug effects; Oxidoreductases - metabolism; Progesterone - blood; Pyrazoles - toxicity; Rats; Rats, Wistar; Reproduction; Reproduction - drug effects; Uterus; Uterus - drug effects; Uterus - metabolism; Uterus - pathology
• ISSN: 0890-6238; 1873-1708
• DOI: 10.1016/j.reprotox.2021.12.009

•Cyantraniliprole induced oxidative stress and damage to the uterine morphology.•Animals treated with both doses had abnormal estrous cycles and reduced number of ovarian follicles compared to control.•Animals that received the 10 mg/kg/day dose showed increased levels of progesterone.•Cyantraniliprole may act as an chemical endocrine disruptor causing female fertility decline. Cyantraniliprole is a synthetic insecticide used to control pests of up to 23 different types of crops. It is able to modulate ryanodine-like calcium channels, which are widely found in the organism of insects and mammals. The objective of this research was to verify the possible reproductive effects of adult female Wistar rats exposure to cyantraniliprole. Animals (67 days old) were exposed to the chemical at doses of 10 or 150 mg/kg/day, orally, for 28 consecutive days (control animals received only the vehicle). Vaginal secretions were collected during the exposure period to assess the regularity of the estrous cycle; the liver, kidneys, pituitary gland, adrenal gland, uterus, and ovaries were collected and weighed; reproductive organs were assessed for histopathological evaluation and for biochemical markers of oxidative stress and progesterone plasma level was measured. Both doses caused negative changes in the morphology and redox system of the uterus and ovaries. Animals exposed to 10 mg/kg also exhibited higher level of plasma progesterone, elevated levels of lipid peroxidation in reproductive organs, increased superoxide dismutase activity in the uterus and glutathione peroxidase activity on the ovary, while the 150 mg/kg group exhibited an increment in estrous cycle length and diminished uterine glandular epithelium. Based on these results, we conclude that cyantraniliprole may have acted as an endocrine disruptor, and its effects are mediated by oxidative stress.