Effect of bile on growth and biofilm formation of non-typhoidal salmonella serovars isolated from seafood and poultry

Bacterial cells adopt various strategies to adapt themselves in diverse environmental conditions. Salmonella is one such bacteria with diverse mechanisms to survive, replicate and infect in wide host range. This study aims at investigating the biofilm-forming ability of multidrug-resistant and sensi...

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Bibliographic Details
Published in:Research in microbiology 2020-07, Vol.171 (5-6), p.165-173
Main Authors: Kotian, Akshatha, Aditya, Vankadari, Jazeela, Kadeeja, Karunasagar, Iddya, Karunasagar, Indrani, Deekshit, Vijaya Kumar
Format: Article
Language:English
Subjects:
Animals
Antimicrobial agents
Antimicrobial resistance
Antimicrobial susceptibility
Bacterial biofilm
Bile
Biofilms - growth & development
Cellulose - biosynthesis
Food Microbiology
Gut
Poultry - microbiology
Salmonella - growth & development
Salmonella - isolation & purification
Salmonella spp
Seafood - microbiology
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Summary:Bacterial cells adopt various strategies to adapt themselves in diverse environmental conditions. Salmonella is one such bacteria with diverse mechanisms to survive, replicate and infect in wide host range. This study aims at investigating the biofilm-forming ability of multidrug-resistant and sensitive Salmonella serovars on exposure to bile. Antibiogram of all the isolates was determined by disk diffusion method and their biofilm-forming ability in the presence or absence of bile was assessed by microtiter plate assay. Biofilm results were validated by calcofluor, Congo red plate and test tube method. Few isolates were selected for further study of their expression of biofilm related genes on exposure to bile using real time PCR. Among the 59 isolates of Salmonella isolated from seafood and poultry, 30 isolates were multi-drug resistant (MDR). Under control conditions, 57% (n = 25) of the serovars were able to form biofilm. While, 86% (n = 51) of the serovars produced biofilm in the presence of bile. The relative gene expression study of the selected serovars for 8 different genes showed a striking difference in the expression levels, supporting the hypothesis that the presence of bile triggers biofilm formation in food associated strains of non-typhoidal Salmonella by upregulation of genes involved in biofilm production.
ISSN:0923-2508
1769-7123
DOI:10.1016/j.resmic.2020.06.002