Study on the inclusion interaction of cucurbit[ n]urils with sanguinarine by spectrofluorimetry and its analytical application

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Molecular and biomolecular spectroscopy, 2010-02, Vol.75 (2), p.912-917
• Main Authors: Li, Chang-Feng, Du, Li-Ming, Zhang, Hui-Ming
• Format: Article
• Language: English
• Subjects: Cucurbit[ n]uril; Inclusion interaction; Sanguinarine; Spectrofluorimetry
• ISSN: 1386-1425
• DOI: 10.1016/j.saa.2009.12.036

The characteristics of host–guest complexation between cucurbit[ n]uril (CB[ n], n = 5, 6, 7, 8) and sanguinarine (SA) were investigated by spectrofluorimetry. The result showed that CB[ n] ( n = 5, 6, 7, 8) reacted with SA to form an inclusion complex. At the optimum reaction conditions, the rectilinear calibration graphs were obtained in the range from 0.1 to 2800 ng mL −1 for the investigated drug, with correlation coefficients 0.9998, 0.9997, 0.9999 and 0.9997 and detection limits 0.052, 0.26, 0.03 and 0.058 ng mL −1 using CB[5], CB[6], CB[7], and CB[8], respectively. The apparent association constants of the complexes were 1.23 × 10 4, 6.73 × 10 3, 4.53 × 10 4 and 1.21 × 10 4 L mol −1, for the reagents in the same order. The result demonstrated that CB[7] exhibited a higher fluorescence signal than CB[5], CB[6], and CB[8]. The proposed method was successfully applied for the determination of the drug in human urine and serum samples.