Synthesis, DNA binding and cleavage activities of copper (II) thiocyanate complex with 4-( N, N-dimethylamino)pyridine and N, N-dimethylformamide

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Molecular and biomolecular spectroscopy, 2011-10, Vol.81 (1), p.21-27
• Main Authors: Chen, Feng-juan, Xu, Min, Xi, Pin-xian, Liu, Hong-yang, Zeng, Zheng-zhi
• Format: Article
• Language: English
• Subjects: 4-( N, N-dimethylamino)pyridine; Cleavage; Copper(II) complex; DNA-binding
• ISSN: 1386-1425
• DOI: 10.1016/j.saa.2011.05.020

[Display omitted] ► The crystal structures of two novel copper(II) thiocyanate complexes with 4-( N, N-dimethylamino) pyridine and N, N-dimethylformamide ( 1) and with4-( N, N-dimethylamino) pyridine ( 2) were obtained. ► The complex ( 1) can bind to calf thymus DNA via partial intercalation mode and cleavage pBR322 DNA. ► Complex 1 could cleavage DNA through a rare hydrolytic pathway. ► We discovered that complex ( 1) has good anti oxidative activity. Two novel copper(II) thiocyanate complexes with 4-( N, N-dimethylamino) pyridine and N, N-dimethylformamide( 1) and with4-( N, N-dimethylamino) pyridine ( 2) have been synthesized and characterized. The crystal and molecular structures of complexes 1 and 2 were determined by single-crystal X-ray diffraction. Antioxidative activity tests in vitro showed that complex 1 has significant antioxidative activity against hydroxyl free radicals from the Fenton reaction and also oxygen free radicals, which is better than standard antioxidants like vitamin C and mannitol. The interaction of complex 1 with calf thymus DNA was investigated by spectroscopic, cyclic voltammetry, and viscosity measurements. Results suggest that complex 1 can bind to DNA via partial intercalation mode. Moreover, complex 1 has been found to cleavage of plasmid DNA pBR322.