Synthesis, spectroscopic, crystal structure and DNA binding of Ru(II) complexes with 2-hydroxy-benzoic acid [1-(4-hydroxy-6-methyl-2-oxo-2H-pyran-3-yl)-ethylidene]-hydrazide

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Molecular and biomolecular spectroscopy, 2011-10, Vol.81 (1), p.128-134
• Main Authors: Chitrapriya, Nataraj, Sathiya Kamatchi, Thangavel, Zeller, Matthias, Lee, Hyosun, Natarajan, Karuppannan
• Format: Article
• Language: English
• Subjects: Crystal structure; DNA binding; Ruthenium(II) complexes; Schiff base; Spectroscopy
• ISSN: 1386-1425
• DOI: 10.1016/j.saa.2011.05.069

Two ruthenium(II) complexes have been synthesized and characterized. The interaction of Ru(II) complexes with Herring sperm DNA was investigated by spectroscopic and viscosity measurements. The experiments suggest that the mode of binding of the complexes to DNA is non-intercalating binder. [Display omitted] ► In this study we synthesized two new Ru(II) complexes. ► They have been characterized by spectroscopic techniques and single crystal XRD. ► Their interaction with DNA was investigated by various spectral studies. Reactions of 2-hydroxy-benzoic acid [1-(4-hydroxy-6-methyl-2-oxo-2H-pyran-3-yl)-ethylidene]-hydrazide (H 2L) with [RuHCl(CO)(EPh 3) 3] (E = P or As) were carried out and the new complexes obtained were characterized by elemental analysis, electronic, IR, 1H NMR and 13C NMR spectroscopic techniques and single crystal X-ray diffraction studies. Complex ( 1) crystallizes in the monoclinic space group P2(1)/ c with unit cell dimensions a = 18.6236(17) Å, b = 12.8627(12) Å, c = 21.683(2) Å, α = 90.00, β = 114.626(2), γ = 90.00 V = 4721.8(8) Å, Z = 4. The crystal structure of the complex shows Ru(II) atom is six-coordinated, forming a slightly distorted octahedral geometry with two P atoms in axial positions, and three chelating donor atoms of the tridentate Schiff base ligand and one carbonyl group located in the equatorial plane. The molecular structure is stabilized by intramolecular O—H···N interactions. No intermolecular hydrogen bond was observed. The intramolecular hydrogen bond exists between the oxygen atom from salicylic acid moiety and nitrogen from the same moiety. A variety of solution studies were carried out for the determination of DNA binding mode of the complexes. The results suggest that both complexes bind to Herring sperm DNA via non intercalative mode.