Three different spectrophotometric methods manipulating ratio spectra for determination of binary mixture of Amlodipine and Atorvastatin

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Molecular and biomolecular spectroscopy, 2011-12, Vol.83 (1), p.140-148
• Main Authors: Darwish, Hany W., Hassan, Said A., Salem, Maissa Y., El-Zeiny, Badr A.
• Format: Article
• Language: English
• Subjects: Atorvastatin and Amlodipine; Derivative ratio; Mean centering; Ratio subtraction; Spectrophotometry
• ISSN: 1386-1425
• DOI: 10.1016/j.saa.2011.08.005

[Display omitted] ► Different spectrophotometric methods handling ratio spectra. ► Simple and rapid without need for sophisticated instruments or expensive solvents. ► Methods validated as per ICH guidelines. ► Accuracy, precision and robustness are found to be within the acceptable limit. Three simple, specific, accurate and precise spectrophotometric methods manipulating ratio spectra are developed for the simultaneous determination of Amlodipine besylate (AM) and Atorvastatin calcium (AT) in tablet dosage forms. The first method is first derivative of the ratio spectra ( 1DD), the second is ratio subtraction and the third is the method of mean centering of ratio spectra. The calibration curve is linear over the concentration range of 3–40 and 8–32 μg/ml for AM and AT, respectively. These methods are tested by analyzing synthetic mixtures of the above drugs and they are applied to commercial pharmaceutical preparation of the subjected drugs. Standard deviation is