FT-IR spectral studies on certain human urinary stones in the patients of rural area

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Molecular and biomolecular spectroscopy, 2012-07, Vol.93, p.260-265
• Main Authors: Selvaraju, R., Thiruppathi, G., Raja, A.
• Format: Article
• Language: English
• Subjects: Calcium oxalate; Carbonate and uric acid; FT-IR; Phosphate; Urinary stones
• ISSN: 1386-1425
• DOI: 10.1016/j.saa.2012.03.028

FT-IR and power X-ray study results indicate that, stones have different composition, i.e., calcium oxalate, calcium phosphate, carbonate apatite and magnesium ammonium phosphate and uric acid. From the spectral, the chemical constituents of urinary stones were identified. The quantitative estimation was performed for four consecutive peaks for calcium oxalate monohydrate (COM), calcium phosphate (apatite), Struvite (MAPH) and uric acid. [Display omitted] ► FT-IR spectra of twenty human urinary stones were analysed. ► The chemical constituents of human urinary stones were identified. ► Qualitative and quantitative estimation of human urinary stones were performed. Fourier transform infrared spectroscopy (FT-IR) has been carried out to analyze the organic and inorganic constituent of human urinary stones. Patient's hailing from Rajah Muthiah Medical College and Hospital, Annamalai University, Tamil Nadu, India was selected for the study. The FT-IR results indicate that stones have different composition, i.e., namely calcium oxalate, calcium phosphate, carbonate apatite and magnesium ammonium phosphate and uric acid. From the spectral and powder X-ray diffraction pattern, the chemical constituents of urinary stones were identified. The quantitative estimations of calcium oxalate monohydrate (COM) 1620cm−1, calcium phosphate (apatite) 1037cm−1, magnesium ammonium phosphate (struvite) 1010cm−1, calcium carbonate 1460cm−1 and uric acid 1441cm−1 were calculated using particular peaks of FT-IR studies. The study reveals that calcium oxalate monohydrate and calcium phosphate type urinary stones were predominant whereas magnesium ammonium phosphate are in moderate level, and calcium carbonate and uric acid are in low. Calcium phosphate is found in all the stones and calcium oxalate monohydrate is found to be higher. Quantitative analyses of urinary stones show that calcium oxalate monohydrate (40%), apatite (30%), magnesium ammonium phosphate (23%) and uric acid (7%) are present in all the urinary stone samples.