Study on the adsorption of DNA on the layered double hydroxides (LDHs)

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Molecular and biomolecular spectroscopy, 2014, Vol.121, p.387-393
• Main Authors: Li, Bin, Wu, Pingxiao, Ruan, Bo, Liu, Paiyu, Zhu, Nengwu
• Format: Article
• Language: English
• Subjects: Adsorption; DNA; Layered double hydroxides; Mechanism
• ISSN: 1386-1425
• DOI: 10.1016/j.saa.2013.10.099

[Display omitted] •Four kinds of layered double hydroxides (LDHs) were synthesized to adsorb the DNA.•We compared the adsorption between different LDHs and discovered the difference.•Discussed the influence of various factors variables on adsorption process.•Some models were used to discuss the adsorption mechanism between LDHs and DNA. Four kinds of layered double hydroxides (LDHs) were prepared by chemical coprecipitation method and used as DNA adsorbents. Multiple characterization tools such as power X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy and Standard electronic modules (SEM) were employed to characterize the LDHs. By examining the effect of initial concentration, solution pH, adsorption experiments were carried out to investigate the adsorption capacities of LDHs for DNA. The results revealed that the LDHs with Mg/Al=3 had higher ability on adsorbing the DNA and were not affected by pH values. The LDHs exhibited excellent adsorption properties and completely adsorbed DNA within 2h. The adsorption equilibrium data were fitted to the Langmuir and Freundlich models, showing that the Langmuir model which represented monolayer adsorption had better correlation with the adsorption linear equation. In addition, Circular dichroism (CD) spectrum, UV–vis spectorscopy and agarose gel electrophoresis revealed the integrity of DNA structure, suggesting that there had no damage on the DNA structure during the adsorption process.