Mononuclear dioxomolybdenum(VI) thiosemicarbazonato complexes: Synthesis, characterization, structural illustration, in vitro DNA binding, cleavage, and antitumor properties

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Molecular and biomolecular spectroscopy, 2015-02, Vol.136, p.1335-1348
• Main Authors: Hussein, Mouayed A., Guan, Teoh S., Haque, Rosenani A., Khadeer Ahamed, Mohamed B., Abdul Majid, Amin M.S.
• Format: Article
• Language: English
• Subjects: Antiproliferative activity; DNA binding; DNA cleavage; Molybdenum(VI) complexes; Thiosemicarbazone; X-ray crystal structure
• ISSN: 1386-1425
• DOI: 10.1016/j.saa.2014.10.021

[Display omitted] •Four Schiff bases derived from 4-ethyl (methyl) thiosemicarbazone were synthesized.•The ligands were coordinated with molybdenum(VI).•The molecular structure of the complexes were characterized by X-ray single crystal.•DNA binding and DNA cleavage by the ligands and complexes were evaluated.•The activity of complexes against human colorectal (HCT 116) cell line was investigated. Four dioxomolybdenum(VI) complexes were synthesized by reacting [MoO2(acac)2] with N-ethyl-2-(5-bromo-2-hydroxybenzylidene) hydrazinecarbothioamide (1), N-ethyl-2-(5-allyl-3-methoxy-2-hydroxybenzylidene) hydrazinecarbothioamide (2), N-methyl-2-(3-tert-butyl-2-hydroxybenzylidene) hydrazinecarbothioamide (3), and N-ethyl-2-(3-methyl-2-hydroxybenzylidene) hydrazinecarbothioamide (4). The molecular structures of 1, 2, and all the synthesized complexes were determined using single crystal X-ray crystallography. The binding properties of the ligand and complexes with calf thymus DNA (CT-DNA) were investigated via UV, fluorescence titrations, and viscosity measurement. Gel electrophoresis revealed that all the complexes cleave pBR 322 plasmid DNA. The cytotoxicity of the complexes were studied against the HCT 116 human colorectal cell line. All the complexes exhibited more pronounced activity than the standard reference drug 5-fluorouracil (IC50 7.3μM). These studies show that dioxomolybdenum(VI) complexes could be potentially useful in chemotherapy.