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Fourier transform infrared spectroscopy based spectral biomarkers of metastasized breast cancer progression

Breast cancer is a global health issue and the second leading cause of cancer death in women. Breast cancer tends to migrate to bone and causes bone metastases which is ultimately the cause of death. Here, we report the use of FTIR to identify spectral biomarkers of cancer progression on 3D in vitro...

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Published in:Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Molecular and biomolecular spectroscopy, 2019-02, Vol.208, p.85-96
Main Authors: Kar, Sumanta, Katti, Dinesh R., Katti, Kalpana S.
Format: Article
Language:English
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Summary:Breast cancer is a global health issue and the second leading cause of cancer death in women. Breast cancer tends to migrate to bone and causes bone metastases which is ultimately the cause of death. Here, we report the use of FTIR to identify spectral biomarkers of cancer progression on 3D in vitro model of breast cancer bone metastasis. Our results indicate that the following spectral biomarkers can monitor cancer progression, for example, lipids (CH2 asymmetric/CH2 symmetric stretch), Amide I/Amide II, and RNA/DNA. Principal component analysis also confirmed the involvement of protein, lipids and nucleic acids in cancer progression on sequential culture. The collective observations from this study suggest successful application of FTIR as a non-invasive and accurate method to identify biochemical changes in cancer cells during the progression of breast cancer bone metastasis. [Display omitted] •An efficient FTIR method proposed for monitoring cancer progression on 3D in vitro model of breast cancer bone metastasis.•Intensity ratios of Lipid, Amide I/Amide II, and RNA/DNA bands identified as potential biomarkers for cancer progression.•Principal component analysis also confirmed involvement of protein, lipids and nucleic acids in cancer progression.•FTIR could serve as a non-invasive and cost-effective tool for clinical diagnosis of breast cancer bone metastasis.
ISSN:1386-1425
DOI:10.1016/j.saa.2018.09.052