Characterization of gastric cells infection by diverse Helicobacter pylori strains through Fourier-transform infrared spectroscopy

The infection of Helicobacter pylori, covering 50% of the world-population, leads to diverse gastric diseases as ulcers and cancer along the life-time of the human host. To promote the discovery of biomarkers of bacterial infection, in the present work, Fourier-transform infrared spectra were acquir...

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Bibliographic Details
Published in:Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Molecular and biomolecular spectroscopy, 2019-03, Vol.210, p.193-202
Main Authors: Marques, Vanda, Cunha, Bernardo, Couto, Andreia, Sampaio, Pedro, Fonseca, Luís P., Aleixo, Sandra, Calado, Cecília R.C.
Format: Article
Language:English
Subjects:
Biomarkers
Gastric adenocarcinoma cells
Helicobacter pylori
Infrared spectroscopy
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Summary:The infection of Helicobacter pylori, covering 50% of the world-population, leads to diverse gastric diseases as ulcers and cancer along the life-time of the human host. To promote the discovery of biomarkers of bacterial infection, in the present work, Fourier-transform infrared spectra were acquired from adenocarcinoma gastric cells, incubated with H. pylori strains presenting different genotypes concerning the virulent factors cytotoxin associated gene A and vacuolating cytotoxin A. Defined absorbance ratios were evaluated by diverse methods of statistical inference, according to the fulfillment of the tests assumptions. It was possible to define from the gastric cells, diverse absorbance ratios enabling to discriminate: i) The infection; ii) the bacteria genotype; and iii) the gastric disease of the patients from which the bacteria were isolated. These biomarkers could fasten the knowledge of the complex infection process while promoting a platform for a new diagnostic method, rapid but also specific and sensitive towards the diagnosis of both infection and bacterial virulence. [Display omitted] •FTIR spectroscopy monitoring of gastric cells infection•Monitoring Helicobacter pylori infection•Discriminate the genotype of H. pylori infecting gastric cells
ISSN:1386-1425
DOI:10.1016/j.saa.2018.11.001