Derivative constant wavelength synchronous fluorescence spectrometry for the simultaneous detection of cefadrine and cefadroxil in water samples

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Molecular and biomolecular spectroscopy, 2020-03, Vol.229, p.117903, Article 117903
• Main Authors: Elbalkiny, Heba T., Yehia, Ali M., Riad, Safa'a M., Elsaharty, Yasser S.
• Format: Article
• Language: English
• Subjects: Cefadroxil; Cefradine and water samples; Derivative synchronous spectrofluorimetry
• ISSN: 1386-1425
• DOI: 10.1016/j.saa.2019.117903

A sensitive accurate spectrofluorimetric technique was developed to detect cefadroxil and cefradine traces in water samples simultaneously, by applying a procedure based on the formation of hydrolysis products corresponding to these compounds by sodium hydroxide (1 N NaOH) treatment. The conventional and the synchronous fluorescence spectra of these hydrolyzed products were totally overlapped making resolving of this mixture impossible. The second-derivative constant-wavelength synchronous fluorescence spectra allowed their detection simultaneously in a single scan after experimental conditions optimization, which was measured at 390 nm and 379 nm for cefadroxil and cefradine, respectively at Δλ = 30.0. The calibration curves between derivative synchronous fluorescence intensity and drugs concentration showed suitable linear correlation in the range of 0.1 to 5 μg.mL−1 for cefadroxil and 0.5–10 μg.mL−1 for cefradine. The proposed fluorimetric method is superior in being simple, environmental friendly and cost effective in comparison to the previously published reported methods. [Display omitted] •Simultaneous detection of cefadroxil and cefradine by spectroflourimetric technique.•Resolving the overlapped spectra by applying second derivative synchronous spectroscopy.•The proposed method was validated in terms of linearity, limits of detection and qualification and precision.•Statistical comparison between the proposed second derivative synchronous Fluorescence Spectroscopy and official methods.•The methods have been effectively applied to water samples collected from nature.