Design a fluorometric aptasensor based on CoOOH nanosheets and carbon dots for simultaneous detection of lysozyme and adenosine triphosphate

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Molecular and biomolecular spectroscopy, 2020-06, Vol.233, p.118197, Article 118197
• Main Authors: Saberi, Zeinab, Rezaei, Behzad, Rezaei, Parisa, Ensafi, Ali Asghar
• Format: Article
• Language: English
• Subjects: Adenosine triphosphate; Aptasensor; Carbon dots; Lysozyme; Nanosheets; Simultaneous detection
• ISSN: 1386-1425
• DOI: 10.1016/j.saa.2020.118197

Simultaneous detection of biomarkers and biomolecules with great analytical performance still is challenging. A simple fluorometric dual-functional aptasensor was designed to detect Lysozyme (LYS) and adenosine triphosphate (ATP) as models of a protein and a small molecule simultaneously. The sensing principle of the aptasensor is based on the interactions between cobalt oxyhydroxide CoOOH nanosheets as fluorescence quencher and carbon dots (CDs) as fluorophores. The aptamer labeled with CDs was able to assemble on CoOOH nanosheets and consequently, the fluorescence signal was quenched. With addition target analytes to the system, the aptamers folded around of targets with a strong and specific affinity. Therefore, the labeled aptamer with CDs was detached from CoOOH nanosheets and the fluorescence signal was restored. The fluorescence spectral overlap of these two CDs is the main limitation for the simultaneous analysis. The least squared support vector machine (LS-SVM) was applied to resolve this problem. Under optimal conditions, when LS-SVR was used, detection limits were found 4.0 and 1.8 nmol L−1 for ATP and LYS. The parallel biosensor is capable of monitoring ATP and LYS levels in the biological samples with satisfactory results. [Display omitted] •A new parallel aptasensor based on fluorescence resonance energy transfer was designed.•Using the carbon dots (CDs) as fluorescent labels and the CoOOH nanosheets as a quencher.•CDs have high quantum yield, rapid and easy synthesis, low toxicity and biocompatibility.•Method was applied for the simultaneous determination of Lysozyme and adenosine triphosphate.•Least square support vector machine was employed to resolve the spectra overlapping.