Raman spectroscopy for the analysis of different exo-polysaccharides produced by bacteria

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Molecular and biomolecular spectroscopy, 2020-08, Vol.237, p.118408, Article 118408
• Main Authors: Tahir, Muhammad, Majeed, Muhammad Irfan, Nawaz, Haq, Ali, Saqib, Rashid, Nosheen, Kashif, Muhammad, Ashfaq, Iram, Ahmad, Waqar, Ghauri, Komal, Sattar, Fazal, Jawad, Iqra, Ghauri, M.A., Anwar, Munir A.
• Format: Article
• Language: English
• Subjects: Bacteria; Exo-polysaccharides; Principal component analysis; Raman spectroscopy
• ISSN: 1386-1425
• DOI: 10.1016/j.saa.2020.118408

In this study, Raman spectroscopy is employed for the characterization and comparison of two different classes of exo-polysaccharides including glucans and fructans which are produced by different bacteria. For this purpose, nine samples are used including five samples of glucans and four of fructans. Raman spectral results of all these polysaccharides show clear differences among various glucans as well as fructans showing the potential of this technique to identify the differences within the same class of the compounds. Moreover, these two classes are also compared on the basis of their Raman spectral data and can be differentiated on the basis of their unique Raman features. Multivariate data analysis techniques, Principle Component Analysis (PCA) is found very helpful for the comparison of the Raman spectral data of these classes of the carbohydrates. [Display omitted] •Raman spectral characterisation of exo-polysaccharides including glucans and fructans which are produced by bacteria.•Raman spectroscopy shows clear differenciation among various glucans as well as fructans.•Principle Component Analysis found helpful for comparison of Raman spectral data of these classes of exo-polysaccharides