The development of lateral flow immunoassay strip tests based on surface enhanced Raman spectroscopy coupled with gold nanoparticles for the rapid detection of soybean allergen β-conglycinin

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Molecular and biomolecular spectroscopy, 2020-11, Vol.241, p.118640, Article 118640
• Main Authors: Xi, Jun, Yu, Qiurong
• Format: Article
• Language: English
• Subjects: Monoclonal antibody; SERS-LFA; β-Conglycinin
• ISSN: 1386-1425
• DOI: 10.1016/j.saa.2020.118640

β-Conglycinin is an important storage protein in soybean, which can potentially cause food allergies in human. In this study, a sensitive mouse monoclonal antibody (3D11 mAb) with a high affinity was prepared, and sandwich lateral flow immunochromatographic detection strips were developed for the rapid detections of the soybean allergen β-conglycinin. The 3D11 mAb was combined with a rabbit polyclonal antibody in order to establish strips. The titer of 3D11 mAb was 1:2.56 × 105. The affinity constant of the 3D11 mAb was 9.6 × 109. The lowest detection limit with the naked eye of the double antibody sandwich strips was 1 μg/mL. In addition, chemical molecules p-aminothiophenol with colloidal gold were used as Raman enhancement signals in order to achieve quantitative detections of the β-conglycinin. It was determined in this study that the practical working range of the β-conglycinin concentrations was between 160 ng/mL and 100 μg/mL with the developed assay. [Display omitted] •A SERS-based lateral flow immunoassay strip test was developed for the rapid simultaneous detection of β-conglycinin.•This assay utilized a pair of antibodies for strip assembling including monoclonal antibody and polyclonal antibody.•The effect of SERS-LFA was verified with the double antibody sandwich ELISA method.•We made some attempts to discover the relationship between PATP signal and gold particles.