A ratiometric fluorescent probe based on quinoline for monitoring and imaging of Leucine aminopeptidase in liver tumor cells

[Display omitted] •A novel galactose-appended quinoline hepatoma-specific ratiometric fluorescent probe(Gal-QL-Leu) for LAP.•Probe Gal-QL-Leu demonstrated a obvious ratiometric characteristics.•Successfully applied for selective imaging of LAP in HepG2, HCT116, A549 and HeLa cells. Leucine aminopept...

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Published in:Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Molecular and biomolecular spectroscopy, 2021-03, Vol.249, p.119328, Article 119328
Main Authors: Du, Kui, Sheng, Li, Luo, Xiang, Fan, Gang, Shen, Dadong, Wu, Chunlei, Shen, Runpu
Format: Article
Language:English
Subjects:
Hepatoma-specific
Leucine aminopeptidase
Liver cancer
Quinoline
Ratiometric
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Summary:[Display omitted] •A novel galactose-appended quinoline hepatoma-specific ratiometric fluorescent probe(Gal-QL-Leu) for LAP.•Probe Gal-QL-Leu demonstrated a obvious ratiometric characteristics.•Successfully applied for selective imaging of LAP in HepG2, HCT116, A549 and HeLa cells. Leucine aminopeptidase (LAP) is known as an important potential biomarker for liver malignancy and it is urgent to develop an intuitive and effective method to monitor the activity of LAP in liver cancer. Although, numerous LAP fluorescent probes had been developed, it is still a challenge to detect LAP activity in liver cancer. Herein, combained with the DFT, we reported a novel galactose-appended hepatoma-specific ratiometric fluorescent probe (Gal-QL-Leu) based on quinoline group for imaging and tracing LAP in liver tumor cells. Probe Gal-QL-Leu demonstrated a obvious ratiometric characteristics, better selectivity, good biocompatibility and high sensitivity. Moreover, the selective imaging of LAP in HepG2, HCT116, A549 and HeLa cells had been achieved with probe Gal-QL-Leu, demonstrating good application prospect in the detection of LAP activity in liver tumor cells.
ISSN:1386-1425
DOI:10.1016/j.saa.2020.119328