Alliance between doping Ag and dual ligands-enhanced fluorescent gold nanoclusters for the assays of vitamin B12 and chlortetracycline hydrochloride

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Molecular and biomolecular spectroscopy, 2021-12, Vol.263, p.120194, Article 120194
• Main Authors: Hu, Yue, Yu, Weihua, Liao, Yunwen, Jiang, Xiaohui, Cheng, Zhengjun
• Format: Article
• Language: English
• Subjects: Assay; Chlortetracycline hydrochloride; Quenching mechanism; TSA/BSA-Au/AgNCs; Vitamin B12
• ISSN: 1386-1425
• DOI: 10.1016/j.saa.2021.120194

Alliance between doping Ag and dual ligands (BSA and TSA)-enhanced fluorescent gold nanoclusters as a probe was prepared by one-step wet chemical synthesis method, and the probe has remarkable selectivity and sensitivity for monitoring VB12/CCH. The fluorescence quenching of the alloy NCs by VB12/CCH comes from Förster resonance energy transfer combined with inner filter effect. [Display omitted] •Enhanced fluorescent AuNCs as a probe was developed.•Discussing effects of doping Ag and dual ligands on the synthesis of AuNCs.•Analyzing fluorescence quenching mechanism of the Au/AgNCs-VB12/CCH system.•The probe was successfully used for monitoring VB12/CCH in real samples. A stable, water-soluble, heightened quantum yields (QYs) Au nanoclusters by the alliance between doping Ag and dual ligands (thiosalicylic acid and bovine serum albumin) (TSA/BSA-Au/AgNCs) was prepared using one-step wet chemical synthesis. The effect of different types of aromatic thiols and the molar ratio of Au-Ag on the photo-luminescence performance of AuNCs was discussed in detail. The alloy NCs is shown to be viable fluorescent method for vitamin B12 (VB12) and chlortetracycline hydrochloride (CCH) assays, and become an excellent temperature sensor in the range of 10–50 °C. The fluorescence (FL) of TSA/BSA-Au/AgNCs was quenched with the addition of VB12 or CCH coming from Förster resonance energy transfer (FRET) combined with inner filter effect (IFE). The method can detect VB12/CCH by fluorometry with a linear response in the range of 0.33–60.0/0.33–60.0 μmol·L−1 and a 71.0/64.0 nmol·L−1 detection limit (at 3σ/slope). Furthermore, the proposed method was extended to the assays of VB12 in mineral water or tablets and CCH in veterinary drug or ointment with satisfactory results.